We have identified a bacterial strain, Paracoccus species 12, which is a producer of a novel restriction endonuclease designated PecI. The enzyme recognizes the palindromic hexanucleotide DNA sequence
5′-TTA↑TAA-3′ and cleaves it to generate blunt ends, as indicated by the arrow. Thus, PecI is a true isoschizomer of the restriction endonuclease PsiI.
Compared to PsiI, PecI offers several advantages, including higher enzyme concentration and 100% activity in ROSE buffer, which enables the availability of a Turbo version for rapid DNA digestion.
The properties of PecI are described in the following publication:
Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Abdurashitov M.A., Belichenko O.A., Degtyarev S.Kh. (2024)
The restriction endonuclease PecI from the bacterial strain Paracoccus species 12 recognizes the DNA sequence 5′-TTA^TAA-3′ and is an isoschizomer of PsiI
DNA-Recognizing Enzymes, 2024(1).
DOI: 10.26213/SE.2024.47.21.001