AccB7 I

1,250.00 ₽ – 5,000.00 ₽

  • CNY: 101.65 ¥ - 406.60 ¥

Restriction endonuclease AccB7 I

  • Recognition site: CCANNNN↑NTGG / GGTN↓NNNNACC
  • Источник: Acinetobacter calcoaceticus B7
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E179 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E179TS200 uTurbo5 000 u/ml1,250.00 ₽
  • CNY: 101.65 ¥
E180TL1000 uTurbo5 000 u/ml5,000.00 ₽
  • CNY: 406.60 ¥
E179S200 uRegular5 000 u/ml1,250.00 ₽
  • CNY: 101.65 ¥
E180L1000 uRegular5 000 u/ml5,000.00 ₽
  • CNY: 406.60 ¥

Description

Recognition site and hydrolysis position:

CCANNNNNTGG CCANNNN↑NTGG
GGTNNNNNACC GGTN↓NNNNACC

Source: Acinetobacter calcoaceticus B7
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dcm-)
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
10 100 25 50 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dcm methylation (CmCWGG): CCANNNCCTGG or CCAGGNNNTGG.
Notes: High enzyme concentration may result in star activity.
References:
Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh., Verhosina, V.A.,Vinogradova, T.P, Izv. Sib. Otd. Akad. Nauk SSSR 1: 35-37 (1990).

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