BstPA I

6,000.00 ₽ – 24,000.00 ₽

  • CNY: 487.92 ¥ - 1,951.68 ¥

Restriction endonuclease BstPA I

  • Recognition site: GACNN↑NNGTC / CTGNN↓NNCAG
  • Источник: Bacillus stearothermophillus PA
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 65
  • Температура инактивации, 20 мин при: N
SKU: SE-E299 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E299S1000 uRegular20 000 u/ml6,000.00 ₽
  • CNY: 487.92 ¥
E300L5000 uRegular20 000 u/ml24,000.00 ₽
  • CNY: 1,951.68 ¥

Description

Recognition site and hydrolysis position:

GACNNNNGTC GACNN↑NNGTC
CTGNNNNCAG CTGNN↓NNCAG

Source: Bacillus stearothermophillus PA
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
50 25 50 50 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme less than 5% of the DNA fragments can be ligated
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 25°C Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration and incubation at 65°C for 16 hours results in star activity.
References:
Tomilova, J.E., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.Kh. Unpublished observations (2000).

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