BstX I

BstX I

Price range: 1 500 ₽ through 6 000 ₽

Restriction endonuclease BstX I

  • Recognition site:
    5'… CCANNNNN▼NTGG …3'
    3'… GGTN▲NNNNNACC …5'
  • Source: Bacillus stearothermophilus X
  • Optimal buffer: SE-buffer O
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E465 Category:

SKUРackagePackageVariantConcentrationPrice 
E465S200 uRegular5 000 u/ml1 500 ₽
E466L1000 uRegular5 000 u/ml6 000 ₽

Description

Recognition site and hydrolysis position:

CCANNNNNNTGG CCANNNNN↑NTGG
GGTNNNNNNACC GGTN↓NNNNNACC

Source: Bacillus stearothermophilus X
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
10 10 100 75 25 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Wise, R., Schildkraut, I. Unpublished observations (1992).

Additional Information

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