BstAU I

BstAU I

Price range: ₽6500,00 through ₽26000,00

Restriction endonuclease BstAU I

  • Recognition site:
    5'… T▼GTACA …3'
    3'… ACATG▲T …5'
  • Источник: Bacillus stearothermophilus AU
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E267 Category:

SKUРackagePackageVariantConcentrationPrice 
E267TS1000 uTurbo20 000 u/ml₽6500,00
E268TL5000 uTurbo20 000 u/ml₽26000,00
E267S1000 uRegular20 000 u/ml₽6500,00
E268L5000 uRegular20 000 u/ml₽26000,00

Description

Recognition site and hydrolysis position:

TGTACA T↑GTACA
ACATGT ACATG↓T

Source: Bacillus stearothermophillus AU
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
10 50 25 100 25 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme > 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Abdurashitov, M.A., Dedkov, V.S., Shinkarenko, N.M., Tomilova, J.E., Degtyarev, S.K. Unpublished observations (2003)

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