Mlu I

Mlu I

Price range: 3 800 ₽ through 15 200 ₽

Restriction endonuclease Mlu I

  • Recognition site:
    5'… A▼CGCGT …3'
    3'… TGCGC▲A …5'
  • Source: Micrococcus luteus
  • Optimal buffer: SE-buffer O
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E085 Category:

SKUРackagePackageVariantConcentrationPrice 
E085S1000 uRegular20 000 u/ml3 800 ₽
E086L5000 uRegular20 000 u/ml15 200 ₽
E085TS1000 uTurbo10 000-30 000 u/ml3 800 ₽
E086TL5000 uTurbo10 000-30 000 u/ml15 200 ₽

Description

Recognition site and hydrolysis position:

ACGCGT A↑CGCGT
TGCGCA TGCGC↓A

Source: Micrococcus luteus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
0 10 100 25 10 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Sugisaki, H., Kanazawa, S. Gene 16: 73-78 (1981).

Additional Information

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