MroX I

1,150.00 ₽ – 4,600.00 ₽

  • CNY: 93.52 ¥ - 374.07 ¥

Restriction endonuclease MroX I

  • Recognition site: GAANN↑NNTTC / CTTNN↓NNAAG
  • Источник: Micrococcus roseus X
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E249 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E249S200 uRegular5 000 u/ml1,150.00 ₽
  • CNY: 93.52 ¥
E250L1000 uRegular5 000 u/ml4,600.00 ₽
  • CNY: 374.07 ¥

Description

Recognition site and hydrolysis position:

GAANNNNTTC GAANN↑NNTTC
CTTNNNNAAG CTTNN↓NNAAG

Source: Micrococcus roseus X
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
50 50 50 100 25 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 50% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Abdurashitov, M.A., Bondar, T.S., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (1996).

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