SmiM I

5,750.00 ₽ – 23,000.00 ₽

  • CNY: 467.59 ¥ - 1,870.36 ¥

Restriction endonuclease SmiM I

  • Recognition site: CAYNN↑NNRTG / GTRNN↓NNYAC
  • Источник: Sphingobacterium mizutae M
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E293 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E293S500 uRegular5 000 u/ml5,750.00 ₽
  • CNY: 467.59 ¥
E294L2500 uRegular5 000 u/ml23,000.00 ₽
  • CNY: 1,870.36 ¥

Description

Recognition site and hydrolysis position:

CAYNNNNRTG CAYNN↑NNRTG
GTRNNNNYAC GTRNN↓NNYAC

Source: Sphingobacterium mizutae M
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
10 10 75 100 10 60

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Dedkov, V.S., Najakshina, T.N., Gushina, E.N., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1999).

Related products