Description
Recognition site and hydrolysis position:
| GCTCCN | GCTCCN↑ |
| CGAGGN | CGAG↓GN |
Source: Lysinibacillus manganicus An22
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 100 | 75 | 50 | 50 | 75 | 80 |
Storage conditions: 20 mM Tris-HCl (pH 7.5); 250 mM NaCl; 100 μg/ml BSA; 7 mM 2-mercaptoethanol, 50% glycerol. Store at -20ºC.
Ligations:
After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37ºC.
Reagents Supplied with Enzyme:
Methylation sensitivity:
Notes:
References:
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