Description
DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3` -> 5` exonuclease activity, but has lost 5` -> 3` exonuclease activity.
Applications:
– dideoxy sequencing;
– polishing ends;
– second strand cDNA synthesis
Source: Isolated from E.coli strain that carries the cloned DNA Polymerase I, Large (Klenow) Fragment gene.
Unit definition: DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3` -> 5` exonuclease activity, but has lost 5` -> 3` exonuclease activity.
Applications:
– dideoxy sequencing;
– polishing ends;
– second strand cDNA synthesis
Reaction buffer: SE-buffer Klenow fragment
Storage conditions: 10 mM KH2PO4 (pH 7.5); 50 mM KCl; 0.5 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Quality control: The enzyme is purified free of contaminating endonucleases andexonucleases.
