SE-buffers activity and inactivation temperature (20 minutes)

Enzyme Recognition sequence Optimal buffer BSA % Activity Optimal temperature, °С Inactivation, °С
B G O W Y Rose
Aat II
GACGT↑C
C↓TGCAG
 Y – 10 – 25 25 – 50 10 – 25 25 – 50 100 50 37 65
Abs I
CC↑TCGAGG
GGAGCT↓CC
 * – 75 – 100 10 – 25 0 – 10 50 – 75 0 – 10 50 37 65
Acc16 I
TGC↑GCA
ACG↓CGT
 W – 50 – 75 75 – 100 25 – 50 100 75 – 100 70 37 65
Acc36 I
ACCTGC(N)4↑
TGGACG(N)8↓
 Y – 25 – 50 25 – 50 50 – 75 50 – 75 100 100 37 65
Acc65 I
G↑GTACC
CCATG↓G
 W – 10 – 25 25 – 50 75 – 100 100 10 – 25 100 37 65
AccB1 I
G↑GYRCC
CCRYG↓G
 K + 50 – 75 10 – 25 10 – 25 75 – 100 50 – 75 30 37 65
AccB7 I
CCANNNN↑NTGG
GGTN↓NNNNACC
 G – 10 – 25 100 25 – 50 50 – 75 50 – 75 100 37 65
AccBS I
GAG↑CGG
CTC↓GCC
 Y – 75 – 100 75 – 100 25 – 50 25 – 50 100 100 37 65
Acl I
AA↑CGTT
TTGC↓AA
 Y + 0 – 10 0 – 10 0 – 10 0 – 10 100 80 37 65
AclW I
GGATC(N)4↑
CCTAG(N)5↓
 Y + 75 – 100 50 – 75 0 – 10 0 – 10 100 30 37 65
Aco I
Y↑GGCCR
RCCGG↓Y
 Y – 50 – 75 75 – 100 50 – 75 25 – 50 100 100 37 65
Acs I
R↑AATTY
YTTAA↓R
 W + 25 – 50 50 – 75 50 – 75 100 10 – 25 100 50 80
Acu I
CTGAAG(N)16↑
GACTTC(N)14↓
 Y+ SAM + 25 – 50 50 – 75 50 – 75 75 – 100 100 50 37 65
Afe I
AGC↑GCT
TCG↓CGA
 Y – 10 – 25 25 – 50 75 – 100 75 – 100 100 100 37 65
Ags I
TTS↑AA
AA↓STT
 Y + 75 – 100 50 – 75 10 – 25 10 – 25 100 50 37 65
Ahl I
A↑CTAGT
TGATC↓A
 B + 100 75 – 100 25 – 50 25 – 50 75 – 100 100 37 N
Ajn I
↑CCWGG
GGWCC↓
 Y – 25 – 50 10 – 25 10 – 25 25 – 50 100 50 55 65
Alu I
AG↑CT
TC↓GA
 Y – 75 – 100 75 – 100 10 – 25 50 – 75 100 50 37 65
AluB I
AG↑CT
TC↓GA
 B + 100 75 – 100 10 – 25 10 – 25 75 – 100 60 37 65
Ama87 I
C↑YCGRG
GRGCY↓C
 W + 10 – 25 50 – 75 75 – 100 100 0 – 10 25 37 65
Apa I
GGGCC↑C
C↓CCGGG
 Y + 50 – 75 25 – 50 0 – 10 0 – 10 100 50 37 65
Ars I
↑(N)8GACNNNNNNTTYG(N)11↑
↓(N)13CTGNNNNNNAARC(N)6↓
 Y + 0 – 10 0 – 10 0 – 10 0 – 10 100 20 30 65
AsiG I
A↑CCGGT
TGGCC↓A
 O – 10 – 25 25 – 50 100 75 – 100 10 – 25 75 37 65
AspA2 I
C↑CTAGG
GGATC↓C
 W + 10 – 25 50 – 75 75 – 100 100 75 – 100 10 37 80
AspLE I
GCG↑C
C↓GCG
 O – 10 – 25 75 – 100 100 50 – 75 25 – 50 100 37 N
AspS9 I
G↑GNCC
CCNG↓G
 W – 50 – 75 50 – 75 75 – 100 100 50 – 75 75 37 65
AsuC2 I
CC↑SGG
GGS↓CC
 Y – 75 – 100 50 – 75 10 – 25 25 – 50 100 40 37 65
AsuHP I
GGTGA(N)8↑
CCACT(N)7↓
 O – 10 – 25 50 – 75 100 75 – 100 25 – 50 100 37 65
AsuNH I
G↑CTAGC
CGATC↓G
 Y + 75 – 100 50 – 75 0 – 10 0 – 10 100 25 37 65
BamH I
G↑GATCC
CCTAG↓G
 G + 25 – 50 100 75 – 100 75 – 100 25 – 50 100 37 65
Bar I
↑(N)7GAAGNNNNNNTAC(N)12↑
↓(N)12CTTCNNNNNNATG(N)7↓
 * – 0 – 10 0 – 10 25 – 50 50 – 75 10 – 25 40 37 65
Bbv12 I
GWGCW↑C
C↓WCGWG
 O – 0 – 10 10 – 25 100 75 – 100 10 – 25 60 37 80
Bgl I
GCCNNNN↑NGGC
CGGN↓NNNNCCG
 2W – 50 – 75 50 – 75 0 – 10 75 – 100 25 – 50 100 37 65
Bgl II
A↑GATCT
TCTAG↓A
 O – 0 – 10 10 – 25 100 25 – 50 10 – 25 100 37 80
Bme18 I
G↑GWCC
CCWG↓G
 O – 10 – 25 25 – 50 100 75 – 100 10 – 25 80 37 65
Bmt I
GCTAG↑C
C↓GATCG
 W – 10 – 25 50 – 75 50 – 75 100 75 – 100 100 37 65
Bmu I
ACTGGG(N)5↑
TGACCC(N)4↓
 Y – 75 – 100 75 – 100 25 – 50 10 – 25 100 25 37 65
BpmI
CTGGAG(N)16↑
GACCTC(N)14↓
 W + 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
Bpu10I
CC↑TNAGC
GGANT↓CG
 K – 10 – 25 25 – 50 50 – 75 50 – 75 25 – 50 100 37 80
Bpu14 I
TT↑CGAA
AAGC↓TT
 G – 50 – 75 100 25 – 50 25 – 50 75 – 100 100 37 65
Bsa29 I
AT↑CGAT
TAGC↓TA
 G + 25 – 50 100 50 – 75 50 – 75 75 – 100 100 37 65
Bsc4 I
CCNNNNN↑NNGG
GGNN↓NNNNNCC
 W + 75 – 100 75 – 100 50 – 75 100 25 – 50 90 55 80
Bse1 I
ACTGGN↑
TGAC↓CN
 Y – 75 – 100 75 – 100 25 – 50 10 – 25 100 100 65 80
Bse118 I
R↑CCGGY
YGGCC↓R
 O – 0 – 10 50 – 75 100 75 – 100 25 – 50 100 65 80
Bse21 I
CC↑TNAGG
GGANT↓CC
 Y – 50 – 75 50 – 75 10 – 25 25 – 50 100 40 37 80
Bse3D I
GCAATGNN↑
CGTTAC↓NN
 G – 10 – 25 100 25 – 50 50 – 75 75 – 100 100 60 80
Bse8 I
GATNN↑NNATC
CTANN↓NNTAG
 G – 25 – 50 100 75 – 100 75 – 100 50 – 75 100 60 80
BseP I
G↑CGCGC
CGCGC↓G
 G – 50 – 75 100 75 – 100 50 – 75 50 – 75 100 50 65
BseX3 I
C↑GGCCG
GCCGG↓C
 O – 10 – 25 25 – 50 100 50 – 75 10 – 25 25 50 80
BslF I
GGGAC(N)10↑
CCCTG(N)14↓
 Y + 25 – 50 25 – 50 10 – 25 25 – 50 100 50 37 80
Bso31 I
GGTCTC(N)1↑
CCAGAG(N)5↓
 O + 25 – 50 75 – 100 100 75 – 100 25 – 50 40 55 80
Bsp13 I
T↑CCGGA
AGGCC↓T
 2K – 25 – 50 50 – 75 75 – 100 50 – 75 0 – 10 5 50 65
Bsp1720 I
GC↑TNAGC
CGANT↓CG
 G – 50 – 75 100 50 – 75 50 – 75 75 – 100 75 37 80
Bsp19 I
C↑CATGG
GGTAC↓C
 2W + 0 – 10 10 – 25 50 – 75 75 – 100 10 – 25 5 37 65
BspAC I
C↑CGC
GGC↓G
 O + 10 – 25 25 – 50 100 75 – 100 10 – 25 100 37 65
BspFN I
CG↑CG
GC↓GC
 Y – 50 – 75 75 – 100 75 – 100 50 – 75 100 100 37 65
BssEC I
C↑CNNGG
GGNNC↓C
 Y – 50 – 75 50 – 75 50 – 75 75 – 100 100 100 60 80
BssNA I
GTA↑TAC
CAT↓ATG
 W + 50 – 75 50 – 75 75 – 100 100 75 – 100 100 37 N
BssT1 I
C↑CWWGG
GGWWC↓C
 2K – 10 – 25 25 – 50 25 – 50 75 – 100 10 – 25 100 60 80
Bst2B I
C↑TCGTG
GAGCA↓C
 Y + 75 – 100 25 – 50 10 – 25 25 – 50 100 100 60 80
Bst2U I
CC↑WGG
GGW↓CC
 G + 75 – 100 100 50 – 75 50 – 75 10 – 25 50 60 N
Bst4C I
ACN↑GT
TG↓NCA
 Y – 75 – 100 75 – 100 10 – 25 25 – 50 100 50 65 N
Bst6 I
CTCTTC(N)1↑
GAGAAG(N)4↓
 Y + 75 – 100 75 – 100 50 – 75 75 – 100 100 100 65 80
BstAC I
GR↑CGYC
CYGC↓RG
 W – 75 – 100 75 – 100 50 – 75 100 75 – 100 100 37 80
BstAF I
C↑TTAAG
GAATT↓C
 W + 10 – 25 25 – 50 75 – 100 100 25 – 50 100 55 80
BstAP I
GCANNNN↑NTGC
CGTN↓NNNNACG
 W – 25 – 50 25 – 50 75 – 100 100 25 – 50 100 60 80
BstAU I
T↑GTACA
ACATG↓T
 W – 10 – 25 50 – 75 25 – 50 100 25 – 50 100 37 80
BstBA I
YAC↑GTR
RTG↓CAY
 W + 25 – 50 25 – 50 75 – 100 100 25 – 50 50 65 80
BstC8I
GCN↑NGC
CGN↓NCG
 Y – 10 – 25 25 – 50 50 – 75 75 – 100 100 100 55 80
BstDE I
C↑TNAG
GANT↓C
 G – 75 – 100 100 25 – 50 50 – 75 10 – 25 100 60 80
BstDS I
C↑CRYGG
GGYRC↓C
 Y – 0 – 10 75 – 100 50 – 75 25 – 50 100 100 65 80
BstEN I
CCTNN↑NNNAGG
GGANNN↓NNTCC
 Y – 50 – 75 50 – 75 25 – 50 25 – 50 100 100 65 80
BstF5 I
GGATGNN↑
CCTAC↓NN
 Y – 75 – 100 50 – 75 25 – 50 50 – 75 100 100 65 80
BstFN I
CG↑CG
GC↓GC
 Y – 75 – 100 50 – 75 25 – 50 25 – 50 100 75 60 80
BstH2 I
RGCGC↑Y
Y↓CGCGR
 Y + 50 – 75 50 – 75 0 – 10 10 – 25 100 100 65 80
BstHH I
GCG↑C
C↓GCG
 Y + 75 – 100 50 – 75 25 – 50 50 – 75 100 100 50 N
BstKTI
GAT↑C
C↓TAG
 W – 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
BstMA I
GTCTC(N)1↑
CAGAG(N)5↓
 W + 25 – 50 50 – 75 50 – 75 100 75 – 100 100 55 65
BstMB I
↑GATC
CTAG↓
 O – 10 – 25 25 – 50 100 75 – 100 10 – 25 100 65 80
BstMC I
CGRY↑CG
GC↓YRGC
 B + 100 75 – 100 10 – 25 10 – 25 50 – 75 100 50 80
BstMW I
GCNNNNN↑NNGC
CGNN↓NNNNNCG
 Y – 10 – 25 25 – 50 25 – 50 50 – 75 100 40 55 80
BstNS I
RCATG↑Y
Y↓GTACR
 B + 100 50 – 75 10 – 25 10 – 25 75 – 100 50 37 65
BstPA I
GACNN↑NNGTC
CTGNN↓NNCAG
 Y – 50 – 75 25 – 50 50 – 75 50 – 75 100 100 65 N
BstSCI
↑CCNGG
GGNCC↓
 Y – 50 – 75 50 – 75 50 – 75 50 – 75 100 60 55 80
BstSF I
C↑TRYAG
GAYRT↓C
 O + 75 – 100 25 – 50 100 50 – 75 50 – 75 100 60 N
BstSL I
GKGCM↑C
C↓MCGKG
 G + 50 – 75 100 50 – 75 75 – 100 75 – 100 100 55 65
BstSN I
TAC↑GTA
ATG↓CAT
 B – 100 50 – 75 0 – 10 10 – 25 50 – 75 50 37 80
BstV1I
GCAGC(N)8↑
CGTCG(N)12↓
 G – 75 – 100 100 75 – 100 75 – 100 75 – 100 100 55 80
BstV2 I
GAAGAC(N)2↑
CTTCTG(N)6↓
 Y + 75 – 100 75 – 100 25 – 50 25 – 50 100 70 55 65
BstX I
CCANNNNN↑NTGG
GGTN↓NNNNNACC
 O – 10 – 25 10 – 25 100 75 – 100 25 – 50 100 37 65
BstX2 I Temporarily unavailable
R↑GATCY
YCTAG↓R
 G – 75 – 100 100 0 – 10 10 – 25 25 – 50 100 60 80
Bsu I
GTATCC(N)6↑
CATAGG(N)5↓
 Y – 75 – 100 50 – 75 10 – 25 25 – 50 100 10 37 65
BsuR I
GG↑CC
CC↓GG
 G – 75 – 100 100 25 – 50 50 – 75 50 – 75 100 37 80
Btr I
CAC↑GTC
GTG↓CAG
 O + 75 – 100 75 – 100 100 75 – 100 75 – 100 100 60 80
Cci I
T↑CATGA
AGTAC↓T
 W + 0 – 10 10 – 25 25 – 50 100 75 – 100 100 55 80
CciN I
GC↑GGCCGC
CGCCGG↓CG
 Y – 25 – 50 50 – 75 75 – 100 75 – 100 100 100 37 65
Dra I
TTT↑AAA
AAA↓TTT
 G + 75 – 100 100 25 – 50 75 – 100 75 – 100 100 37 65
DraIII
CACNNN↑GTG
GTG↓NNNCAC
 2K + 25 – 50 50 – 75 75 – 100 75 – 100 50 – 75 100 37 65
Dri I
GACNNN↑NNGTC
CTGNN↓NNNCAG
 Y – 75 – 100 75 – 100 10 – 25 10 – 25 100 40 37 65
DseD I
GACNNNN↑NNGTC
CTGNN↓NNNNCAG
 Y + 75 – 100 75 – 100 25 – 50 50 – 75 100 30 37 80
EcoICR I
GAG↑CTC
CTC↓GAG
 G + 75 – 100 100 0 – 10 0 – 10 75 – 100 5 37 65
EcoR I
G↑AATTC
CTTAA↓G
 * + 50 – 75 75 – 100 75 – 100 75 – 100 50 – 75 50 37 65
EcoR V
GAT↑ATC
CTA↓TAG
 W + 0 – 10 25 – 50 50 – 75 100 25 – 50 50 37 80
Ege I
GGC↑GCC
CCG↓CGG
 B + 100 75 – 100 10 – 25 50 – 75 75 – 100 100 37 65
Erh I
C↑CWWGG
GGWWC↓C
 2W + 10 – 25 25 – 50 50 – 75 75 – 100 10 – 25 100 37 65
FaeI
CATG↑
↓GTAC
 * + 25 – 50 50 – 75 10 – 25 10 – 25 75 – 100 100 37 65
FaiI
YA↑TR
RT↓AY
 B – 100 50 – 75 10 – 25 25 – 50 25 – 50 100 50 80
FalI
↑(N)8AAGNNNNNCTT(N)13↑
↓(N)13TTCNNNNNGAA(N)8↓
 W+ SAM – 0 – 10 25 – 50 75 – 100 100 50 – 75 70 37 65
FatI
↑CATG
GTAC↓
 G – 10 – 25 100 25 – 50 10 – 25 50 – 75 100 55 65
Fau I
CCCGC(N)4↑
GGGCG(N)6↓
 B – 100 25 – 50 0 – 10 0 – 10 50 – 75 50 55 65
FauND I
CA↑TATG
GTAT↓AC
 Y + 50 – 75 75 – 100 10 – 25 50 – 75 100 100 37 65
Fbl I
GT↑MKAC
CAKM↓TG
 Y – 50 – 75 75 – 100 0 – 10 50 – 75 100 100 55 80
Fok I
GGATG(N)9↑
CCTAC(N)13↓
 Y – 50 – 75 50 – 75 25 – 50 25 – 50 100 100 37 65
FriO I
GRGCY↑C
C↓YCGRG
 Y + 75 – 100 75 – 100 10 – 25 0 – 10 100 25 37 65
Fsp4H I
GC↑NGC
CGN↓CG
 Y – 50 – 75 75 – 100 10 – 25 25 – 50 100 100 37 65
Gsa I
CCCAG↑C
G↓GGTCG
 W + 10 – 25 25 – 50 75 – 100 100 75 – 100 100 70 N
Hae III
GG↑CC
CC↓GG
 G – 75 – 100 100 25 – 50 50 – 75 50 – 75 100 37 80
Hga I
GACGC(N)5↑
CTGCG(N)10↓
 B – 100 75 – 100 10 – 25 25 – 50 50 – 75 50 37 65
Hind II
GTY↑RAC
CAR↓YTG
 G + 75 – 100 100 25 – 50 25 – 50 75 – 100 50 37 65
Hind III
A↑AGCTT
TTCGA↓A
 W + 10 – 25 25 – 50 0 – 10 100 0 – 10 100 37 80
Hinf I
G↑ANTC
CTNA↓G
 O – 25 – 50 75 – 100 100 75 – 100 75 – 100 100 37 80
Hpa I
GTT↑AAC
CAA↓TTG
 Y – 0 – 10 50 – 75 10 – 25 25 – 50 100 25 37 65
Hpa II
C↑CGG
GGC↓C
 B – 100 50 – 75 10 – 25 25 – 50 50 – 75 50 37 80
HpySE526 I
A↑CGT
TGC↓A
 Y – 75 – 100 75 – 100 10 – 25 25 – 50 100 50 37 65
HspA I
G↑CGC
CGC↓G
 Y – 50 – 75 50 – 75 25 – 50 25 – 50 100 100 37 80
Kpn I
GGTAC↑C
C↓CATGG
 B + 100 25 – 50 25 – 50 25 – 50 75 – 100 50 37 80
Ksp22 I
T↑GATCA
ACTAG↓T
 2K + 50 – 75 75 – 100 50 – 75 50 – 75 25 – 50 100 37 65
Kzo9 I
↑GATC
CTAG↓
 G – 50 – 75 100 50 – 75 50 – 75 50 – 75 100 37 65
Lmn I
GCTCCN↑
CGAG↓GN
 B – 100 75 – 100 50 – 75 50 – 75 75 – 100 80 37 65
Mab I
A↑CCWGGT
TGGWCC↓A
 W + 25 – 50 50 – 75 75 – 100 100 50 – 75 100 37 65
Mbo II
GAAGA(N)8↑
CTTCT(N)7↓
 Y – 75 – 100 75 – 100 25 – 50 50 – 75 100 50 37 65
Mfe I
C↑AATTG
GTTAA↓C
 B + 100 75 – 100 10 – 25 25 – 50 75 – 100 25 37 N
Mhl I
GDGCH↑C
C↓HCGDG
 W – 10 – 25 25 – 50 75 – 100 100 10 – 25 100 37 80
Mlu I
A↑CGCGT
TGCGC↓A
 O – 0 – 10 10 – 25 100 25 – 50 10 – 25 50 37 65
Mly113 I
GG↑CGCC
CCGC↓GG
 B – 100 25 – 50 10 – 25 10 – 25 50 – 75 100 37 65
Mnl I
CCTC(N)7↑
GGAG(N)6↓
 G + 75 – 100 100 25 – 50 25 – 50 75 – 100 100 37 65
Mox20 I
TGG↑CCA
ACC↓GGT
 O – 10 – 25 25 – 50 100 75 – 100 25 – 50 75 37 N
MroN I
G↑CCGGC
CGGCC↓G
 B – 100 50 – 75 10 – 25 0 – 10 10 – 25 5 37 80
MroX I
GAANN↑NNTTC
CTTNN↓NNAAG
 W – 50 – 75 50 – 75 50 – 75 100 25 – 50 100 37 65
Msp I
C↑CGG
GGC↓C
 B – 100 75 – 100 50 – 75 75 – 100 75 – 100 100 37 65
MspA1 I
CMG↑CKG
GKC↓GMC
 Y + 10 – 25 75 – 100 10 – 25 25 – 50 100 100 37 65
MspR9 I
CC↑NGG
GGN↓CC
 O – 50 – 75 50 – 75 100 50 – 75 50 – 75 100 37 80
Nru I
TCG↑CGA
AGC↓GCT
 W – 0 – 10 10 – 25 75 – 100 100 10 – 25 100 37 80
PalA I
GG↑CGCGCC
CCGCGC↓GG
 Y – 25 – 50 10 – 25 75 – 100 100 100 40 37 65
Pce I
AGG↑CCT
TCC↓GGA
 Y – 75 – 100 75 – 100 50 – 75 25 – 50 100 100 50 80
Pci I
A↑CATGT
TGTAC↓A
 O – 50 – 75 75 – 100 100 75 – 100 50 – 75 50 37 65
PciS I
GCTCTTCN↑
CGAGAAG(N)4↓
 B – 100 50 – 75 0 – 10 0 – 10 75 – 100 50 37 65
Pct I
GAATGCN↑
CTTAC↓GN
 O – 25 – 50 50 – 75 100 75 – 100 10 – 25 50 37 65
Ple19 I
CGAT↑CG
GC↓TAGC
 Y – 75 – 100 75 – 100 25 – 50 25 – 50 100 100 37 65
Pps I
GAGTC(N)4↑
CTCAG(N)5↓
 Y + 50 – 75 10 – 25 0 – 10 25 – 50 100 50 37 65
Psi I
TTA↑TAA
AAT↓ATT
 B – 100 25 – 50 10 – 25 25 – 50 75 – 100 40 37 65
Psp124B I
GAGCT↑C
C↓TCGAG
 G – 75 – 100 100 10 – 25 0 – 10 75 – 100 30 37 80
Psp6 I
↑CCWGG
GGWCC↓
 B – 100 50 – 75 10 – 25 25 – 50 75 – 100 10 55 80
PspC I
CAC↑GTG
GTG↓CAC
 B + 100 50 – 75 0 – 10 0 – 10 50 – 75 5 37 65
PspE I
G↑GTNACC
CCANTG↓G
 B – 100 50 – 75 25 – 50 50 – 75 50 – 75 100 37 65
PspL I
C↑GTACG
GCATG↓C
 Y + 75 – 100 75 – 100 25 – 50 10 – 25 100 100 37 65
PspN4 I
GGN↑NCC
CCN↓NGG
 Y – 10 – 25 10 – 25 10 – 25 25 – 50 100 50 37 65
PspOM I
G↑GGCCC
CCCGG↓G
 Y – 75 – 100 10 – 25 0 – 10 0 – 10 100 25 37 65
PspPP I
RG↑GWCCY
YCCWG↓GR
 Y – 10 – 25 10 – 25 0 – 10 0 – 10 100 100 37 65
PspX I
VC↑TCGAGB
BGAGCT↓CV
 Y + 50 – 75 50 – 75 25 – 50 75 – 100 100 25 37 80
Psr I
↑(N)7GAACNNNNNNTAC(N)12↑
↓(N)12CTTGNNNNNNATG(N)7↓
 Y + 10 – 25 10 – 25 0 – 10 0 – 10 100 30 30 65
Pst I
CTGCA↑G
G↓ACGTC
 O + 10 – 25 25 – 50 100 25 – 50 25 – 50 50 37 80
PstN I
CAGNNN↑CTG
GTC↓NNNGAC
 Y – 25 – 50 50 – 75 10 – 25 25 – 50 100 100 37 65
Pvu II
CAG↑CTG
GTC↓GAC
 G + 25 – 50 100 25 – 50 25 – 50 25 – 50 100 37 80
Rga I
GCGAT↑CGC
CGC↓TAGCG
 Y – 75 – 100 50 – 75 10 – 25 25 – 50 100 100 55 80
Rig I
GGCCGG↑CC
CC↓GGCCGG
 * + 75 – 100 50 – 75 0 – 10 10 – 25 50 – 75 10 37 65
Rsa I
GT↑AC
CA↓TG
 B – 100 50 – 75 0 – 10 50 – 75 75 – 100 50 37 N
RsaN I
G↑TAC
CAT↓G
 B – 100 75 – 100 50 – 75 50 – 75 75 – 100 100 37 80
Rsr2 I
CG↑GWCCG
GCCWG↓GC
 Y + 50 – 75 75 – 100 0 – 10 10 – 25 100 25 37 65
Sal I
G↑TCGAC
CAGCT↓G
 O – 0 – 10 10 – 25 100 25 – 50 0 – 10 5 37 65
Sbf I
CCTGCA↑GG
GG↓ACGTCC
 Y – 75 – 100 50 – 75 0 – 10 0 – 10 100 50 37 80
Set I
ASST↑
↓TSSA
 Y – 25 – 50 25 – 50 75 – 100 75 – 100 100 100 55 80
SfaN I
GCATC(N)5↑
CGTAG(N)9↓
 O – 10 – 25 25 – 50 100 75 – 100 0 – 10 25 37 80
Sfi I
GGCCNNNN↑NGGCC
CCGGN↓NNNNCCGG
 G + 75 – 100 100 25 – 50 25 – 50 25 – 50 75 50 65
Sfr274 I
C↑TCGAG
GAGCT↓C
 B – 100 75 – 100 50 – 75 50 – 75 75 – 100 100 50 65
Sfr303 I
CCGC↑GG
GG↓CGCC
 B – 100 50 – 75 10 – 25 10 – 25 75 – 100 100 37 65
Sma I
CCC↑GGG
GGG↓CCC
 Y – 0 – 10 0 – 10 0 – 10 0 – 10 100 50 25 65
Smi I
ATTT↑AAAT
TAAA↓TTTA
 O + 25 – 50 25 – 50 100 75 – 100 25 – 50 10 37 65
SmiM I
CAYNN↑NNRTG
GTRNN↓NNYAC
 W – 10 – 25 10 – 25 75 – 100 100 10 – 25 60 37 65
Sph I
GCATG↑C
C↓GTACG
 G + 25 – 50 100 75 – 100 75 – 100 50 – 75 100 37 65
Sse9 I
↑AATT
TTAA↓
 B + 100 75 – 100 50 – 75 50 – 75 75 – 100 75 55 65
Ssp I
AAT↑ATT
TTA↓TAA
 K + 75 – 100 50 – 75 25 – 50 50 – 75 75 – 100 100 37 65
Taq I
T↑CGA
AGC↓T
 Y + 50 – 75 75 – 100 75 – 100 50 – 75 100 100 65 80
Tru9 I
T↑TAA
AAT↓T
 W – 75 – 100 25 – 50 25 – 50 100 50 – 75 100 65 80
TseF I
↑GTSAC
CASTG↓
 B – 100 50 – 75 0 – 10 25 – 50 50 – 75 50 65 N
Tth111 I
GACN↑NNGTC
CTGNN↓NCAG
 Y – 75 – 100 50 – 75 10 – 25 10 – 25 100 100 65 80
Vne I
G↑TGCAC
CACGT↓G
 O – 10 – 25 25 – 50 100 25 – 50 25 – 50 100 37 65
Vsp I
AT↑TAAT
TAAT↓TA
 W – 0 – 10 10 – 25 50 – 75 100 25 – 50 50 37 65
Xba I
T↑CTAGA
AGATC↓T
 O + 75 – 100 75 – 100 100 50 – 75 75 – 100 25 37 65
Xma I
C↑CCGGG
GGGCC↓C
 Y – 75 – 100 50 – 75 0 – 10 0 – 10 100 50 37 65
ZraI
GAC↑GTC
CTG↓CAG
 B – 100 50 – 75 25 – 50 25 – 50 75 – 100 100 37 80
Zrm I
AGT↑ACT
TCA↓TGA
 Y + 50 – 75 25 – 50 0 – 10 0 – 10 100 50 37 65
Zsp2 I
ATGCA↑T
T↓ACGTA
 B + 100 50 – 75 25 – 50 25 – 50 25 – 50 50 37 65