AccB7 I

Enzyme nameAccB7 I
PrototypePflMI
Known analogsPflBI, PflMI, Van91I
SKUSE-E179
Turbo versionAvailable
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CCANNNN▼NTGG …3'
3'… GGTN▲NNNNACC …5'
SourceAcinetobacter calcoaceticus B7
Optimal bufferSE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
10100255050100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA (dcm-)
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityBlocked by overlapping Dcm methylation (C m CWGG) CCANNN CCTGG or CCAGG NNNTGG.
Reagents supplied with enzyme10 X SE-buffer G
NotesHigh enzyme concentration may result in star activity.
References

No publications found.

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