AccB7 I

Enzyme name AccB7 I
Prototype PflMI
Known analogs PflBI, PflMI, Van91I
SKU SE-E179
Turbo version Available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CCANNNN▼NTGG …3'
3'… GGTN▲NNNNACC …5'
Source Acinetobacter calcoaceticus B7
Optimal buffer SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10100255050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dcm methylation (C m CWGG) CCANNN CCTGG or CCAGG NNNTGG.
Reagents supplied with enzyme 10 X SE-buffer G
Notes High enzyme concentration may result in star activity.
References

No publications found.
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