AsiS I

Enzyme nameAsiS I
Prototype#Н/Д
Known analogsRga I, SgfI
SKUSE-E159
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GCGAT▼CGC …3'
3'… CGC▲TAGCG …5'
SourceAn E.coli strain that carries the cloned AsiSI gene from Arthrobacter species S
Optimal bufferSE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
100750102525
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onAdenovirus-2 DNA
Storage conditions20 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter digestion with a 5-fold excess of enzyme, more than 90% of DNA fragments can be ligated by DNA ligase and can be recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityNot blocked by overlapping Dam-methylation (G m ATC): GC GATC GC Blocked by CpG methylation.
Reagents supplied with enzyme10 X SE-buffer B.
NotesHigh enzyme concentration may result in star activity.
References
  1. Unpublished data Abdurashitov M.A., Shinkarenko N.M., Shevchenko A.V., Dedkov V.S., Degtyarev S.Kh Unpublished data. 2000.
// Код для некролога