Bme18 I

Enzyme nameBme18 I
PrototypeAvaII
Known analogsAvaII, Eco47I, SinI, VpaK11BI
SKUSE-E029
Turbo versionAvailable
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… G▼GWCC …3'
3'… CCWG▲G …5'
SourceBacillus megaterium 18
Optimal bufferSE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
1025100751080
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityCleaved of DNA is impaired by overlapping dcm-methylation (C m CWGG) GGW CC WGG.
Reagents supplied with enzyme10 X SE-buffer O
References
  2. S.Kh. Degtyarev, N.I. Rechkunova, A.A. Grinev, V.S. Dedkov Discovery and substrate specificity determination of restriction endonucleases Bme18I and Kzo9I. // Izvestia SB SA USSR, Biological sciences series, No.3, 25-26 (1989). (In Russian)
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