Bse118 I

Enzyme nameBse118 I
PrototypeCfr10I
Known analogsBsrFI, BssAI, Cfr10I
SKUSE-E039
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… R▼CCGGY …3'
3'… YGGCC▲R …5'
SourceBacillus stearothermophilus 118
Optimal bufferSE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature65 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
0501007525100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM KH 2 PO 4 (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer O
References

No publications found.

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