Bse118 I

Enzyme name Bse118 I
Prototype Cfr10I
Known analogs BsrFI, BssAI, Cfr10I
SKU SE-E039
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… R▼CCGGY …3'
3'… YGGCC▲R …5'
Source Bacillus stearothermophilus 118
Optimal buffer SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
0501007525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM KH 2 PO 4 (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer O
Notes
References

No publications found.
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