Bse21 I

Enzyme nameBse21 I
PrototypeSauI
Known analogsAxyI, Bsu36I, Eco81I, SauI
SKUSE-E037
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CC▼TNAGG …3'
3'… GGANT▲CC …5'
SourceBacillus species 21
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
5050102510040
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA (HindIII-digest)
Storage conditions10 mM KH 2 PO 4 (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 2-fold overdigestion with enzyme about 50% of DNA fragments can be ligated by using of high concentration T4 DNA ligase with presence of 10% PEG. Of these more than 90% can be recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
References
  2. V.S. Dedkov, S.Kh. Degtyarev Actinobacillus and Streptococcus: producers of isoschizomers of the restriction endonucleases R.Hph I, R.Sau I, R.Nhe I, R.Mbo I and R.Swa I. // Biol. Chem., Vol. 379, 573-574, (1998).
  3. S.Kh. Degtyarev, A.A. Kolykhalov, V.E. Repin, N.I. Rechkunova, S.V. Netesov Specificity determination of restriction endonuclease Bse21I. // Izvestia SB SA USSR, Biological sciences series, Vol.1, 138-139 (1990). (In Russian)
  4. С.Х. Дегтярев, В.Е. Репин, Н.И. Речкунова, Л.С. Шевченко, Е.П. Иванова, В.А. Рассказов Bacillus species 21 - a strain producer of restriction endonuclease Bse21I. // Soviet Union patent SU 1518372 (1989). (In Russian)
  5. V.E. Repin, S.V. Polshina, N.A. Bozhko, S.Kh. Degtyarev The cultivation of Bacillus species 21 - Bse21I restriction endonuclease producer. // Izvestia SB SA USSR, Biology sciences seria, Molecular byology, vol.1, 108-111 (1989). (In Russian)
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