BseP I

Enzyme name BseP I
Prototype BsePI
Known analogs BssHII, PauI
SKU SE-E181
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… G▼CGCGC …3'
3'… CGCGC▲G …5'
Source Bacillus stearothermophilus P
Optimal buffer SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature 50 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50100755050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity Blocked by CG methylation.
Reagents supplied with enzyme 10 X SE-buffer G
Notes
References
  1. Unpublished data Langdale, J.A., Myers, P.A., Roberts, R.J Unpublished observations (1992).
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