BseP I

Enzyme nameBseP I
PrototypeBsePI
Known analogsBssHII, PauI
SKUSE-E181
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… G▼CGCGC …3'
3'… CGCGC▲G …5'
SourceBacillus stearothermophilus P
Optimal bufferSE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature50 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
50100755050100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivityBlocked by CG methylation.
Reagents supplied with enzyme10 X SE-buffer G
References
  1. Unpublished data Langdale, J.A., Myers, P.A., Roberts, R.J Unpublished observations (1992).
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