BssT1 I

Enzyme nameBssT1 I
PrototypeStyI
Known analogsEco130I, EcoT14I, Erh I, StyI
SKUSE-E207
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… C▼CWWGG …3'
3'… GGWWC▲C …5'
SourceBacillus stearothermophilus T1
Optimal bufferSE-buffer 2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)
Optimal temperature60 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
1025257510100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer 2K
NotesHigh enzyme concentration may result in star activity
References
  1. Unpublished data Serov, G.D., Tereshenko, T.A., Puchkova, L.I., Rechkunova N.I., Degtyarev, S.K Unpublished observations.
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