Bst4C I

Enzyme nameBst4C I
PrototypeTsp4CI
Known analogsHpyCH4III, TaaI, Tsp4CI
SKUSE-E265
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… ACN▼GT …3'
3'… TG▲NCA …5'
SourceBacillus stearothermophilus 4C
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature65 °C
Inactivation temperatureне инактивируется
Buffer activity
BGOWYROSE
7575102510050
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme ~50% of DNA fragments can be ligated.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
References
  1. Unpublished data Shinkarenko, N.M., Schevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K Unpublished observations (1997).
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