BstH2 I

Enzyme nameBstH2 I
PrototypeHaeII
Known analogsBsp143II, HaeII
SKUSE-E171
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… RGCGC▼Y …3'
3'… Y▲CGCGR …5'
SourceBacillus stearothermophilus H2
Optimal bufferSE-buffer Y + BSA
Optimal temperature65 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
5050010100100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM KH 2 PO 4 (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y, BSA
NotesHigh enzyme concentration may result in star activity To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Unpublished data Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K Unpublished observations (1994)
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