Unpublished data
Unpublished observations. (2004)
BstMB I
| Enzyme name | BstMB I | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Prototype | MboI | ||||||||||||
| Known analogs | BfuCI, Bsp143I, DpnII, Kzo9 I, MboI, NdeII, Sau3AI, BstKT I | ||||||||||||
| SKU | SE-E119 | ||||||||||||
| Turbo version | Not available | ||||||||||||
| High-concentration version | Not available | ||||||||||||
| Restriction type | Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites. | ||||||||||||
| Recognition site | 5'… ▼GATC …3' 3'… CTAG▲ …5' | ||||||||||||
| Source | Bacillus stearothermophillus MB | ||||||||||||
| Optimal buffer | SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) | ||||||||||||
| Optimal temperature | 65 °C | ||||||||||||
| Inactivation temperature | 80 °C | ||||||||||||
| Buffer activity |
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| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 65°C in a total reaction volume of 50 μl. | ||||||||||||
| Assayed on | Lambda DNA (dam-) | ||||||||||||
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA. Store at -20°C. | ||||||||||||
| Ligation | After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut | ||||||||||||
| Nonspecific hydrolysis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C | ||||||||||||
| Methylation sensitivity | Blocked by overlapping Dam methylation(G m ATC) GATC. Not blocked by CG methylation. Not cut hemimethylated site:5`- G(6mA)TC-3` / 3`-CTAG-5` | ||||||||||||
| Reagents supplied with enzyme | 10 X SE-buffer O | ||||||||||||
| References |