BstMB I

Enzyme name BstMB I
Prototype MboI
Known analogs BfuCI, Bsp143I, DpnII, Kzo9 I, MboI, NdeII, Sau3AI, BstKT I
SKU SE-E119
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… ▼GATC …3'
3'… CTAG▲ …5'
Source Bacillus stearothermophillus MB
Optimal buffer SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10251007510100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C
Methylation sensitivity Blocked by overlapping Dam methylation(G m ATC) GATC. Not blocked by CG methylation. Not cut hemimethylated site:5`- G(6mA)TC-3` / 3`-CTAG-5`
Reagents supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Unpublished data Kovalenko, K., Kileva, E.V., Mezentseva, N.V., Mikhnenkova, N.A., Dedkov, V.S., Degtyarev, S.K Unpublished observations. (2004)
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