BstMW I

Enzyme name BstMW I
Prototype MwoI
Known analogs HpyF10VI, MwoI
SKU SE-E459
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GCNNNNN▼NNGC …3'
3'… CGNN▲NNNNNCG …5'
Source Bacillus stearothermophilus MW
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1025255010040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Storage at -20°C. Storage at -70°C is recommended for periods longer than 7 days.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y
Notes At 37°C activity is 20% from maximum
References
  1. Unpublished data Nadeev A.N., Dedkov, V.S., Tomilova J.E., Degtyarev, S.K Unpublished observations (2002)
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