BstMW I

Enzyme nameBstMW I
PrototypeMwoI
Known analogsHpyF10VI, MwoI
SKUSE-E459
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GCNNNNN▼NNGC …3'
3'… CGNN▲NNNNNCG …5'
SourceBacillus stearothermophilus MW
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature55 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
1025255010040
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Storage at -20°C. Storage at -70°C is recommended for periods longer than 7 days.
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
NotesAt 37°C activity is 20% from maximum
References
  1. Unpublished data Nadeev A.N., Dedkov, V.S., Tomilova J.E., Degtyarev, S.K Unpublished observations (2002)
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