BstPA I

Enzyme nameBstPA I
PrototypePshAI
Known analogsBoxI, PshAI
SKUSE-E299
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GACNN▼NNGTC …3'
3'… CTGNN▲NNCAG …5'
SourceBacillus stearothermophillus PA
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature65 °C
Inactivation temperatureне инактивируется
Buffer activity
BGOWYROSE
50255050100100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme less than 5% of the DNA fragments can be ligated
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 25°C
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
NotesHigh enzyme concentration and incubation at 65°C for 16 hours results in star activity.
References
  1. Unpublished data Tomilova, J.E., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.Kh Unpublished observations (2000)
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