BstSC I

Enzyme name BstSC I
Prototype ScrFI
Known analogs BssKI, StyD4I, Bme1390I, MspR9 I, ScrFI
SKU SE-E307
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… ▼CCNGG …3'
3'… GGNCC▲ …5'
Source Bacillus stearothermophilus SC
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5050505010060
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity Blocked by Dcm-methylation (C m CWGG) CCWGG.
Reagents supplied with enzyme 10 X SE-buffer Y
Notes At 37°C activity is 10% from maximum.
References
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