BstSC I

Enzyme nameBstSC I
PrototypeScrFI
Known analogsBssKI, StyD4I, Bme1390I, MspR9 I, ScrFI
SKUSE-E307
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… ▼CCNGG …3'
3'… GGNCC▲ …5'
SourceBacillus stearothermophilus SC
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature55 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
5050505010060
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed onLambda DNA (dcm-)
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivityBlocked by Dcm-methylation (C m CWGG) CCWGG.
Reagents supplied with enzyme10 X SE-buffer Y
NotesAt 37°C activity is 10% from maximum.
References
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