DseD I

Enzyme name DseD I
Prototype DrdI
Known analogs AasI, DrdI
SKU SE-E241
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GACNNNN▼NNGTC …3'
3'… CTGNN▲NNNNCAG …5'
Source Deinococcus species D2
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
7575255010030
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml Do not use BSA for long incubation.
References
  1. Unpublished data Abdurashitov, M.A., Kileva, E.V., Dedkov, V.S., Degtyarev, S.K Unpublished observations (1996)
// Код для некролога