Erh I

Enzyme name Erh I
Prototype StyI
Known analogs BssT1 I, Eco130I, EcoT14I, StyI
SKU SE-E061
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… C▼CWWGG …3'
3'… GGWWC▲C …5'
Source Erwinia rhapontici
Optimal buffer SE-buffer 2W (20 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1025507510100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer 2W, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. High enzyme concentration may result in star activity
References
  1. Unpublished data Zhilkina, O.A., Rechkunova, N.I., Semenchenko, G.V., Degtyarev, S.K Unpublished observations (1994)
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