Fbl I

Enzyme nameFbl I
PrototypeAccI
Known analogsAccI, XmiI
SKUSE-E271
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GT▼mKAC …3'
3'… CAKm▲TG …5'
SourceAn E.coli strain that carries the cloned Fbl I gene from Flavobacterium balustinum
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature55 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
5075050100100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, and 50% glycerol; Store at -20°C.
LigationAfter 2-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
References

No publications found.

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