Hpa II

Enzyme name Hpa II
Prototype HpaII
Known analogs BsiSI, HapII, Msp I
SKU SE-E161
Turbo version Available
High-concentration version Not available
Restriction type Type IIEiType IIE enzymes interact with two recognition sites: one site is cleaved, while the second site acts as an allosteric effector site., Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… C▼CGG …3'
3'… GGC▲C …5'
Source An E.coli strain, that carries the cloned gene Hpa II from Haemophilus parainfluenzae
Optimal buffer SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1005010255050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation.
Reagents supplied with enzyme 10 X SE-buffer B
Notes
References
  3. External publication Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973).
// Код для некролога