Hpa II

Enzyme nameHpa II
PrototypeHpaII
Known analogsBsiSI, HapII, Msp I
SKUSE-E161
Turbo versionAvailable
High-concentration versionNot available
Restriction typeType IIEiType IIE enzymes interact with two recognition sites: one site is cleaved, while the second site acts as an allosteric effector site., Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… C▼CGG …3'
3'… GGC▲C …5'
SourceAn E.coli strain, that carries the cloned gene Hpa II from Haemophilus parainfluenzae
Optimal bufferSE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
1005010255050
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityBlocked by CG methylation.
Reagents supplied with enzyme10 X SE-buffer B
References
  3. External publication Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973).
// Код для некролога