Kzo9 I

Enzyme name Kzo9 I
Prototype MboI
Known analogs BfuCI, Bsp143I, BstMB I, DpnII, MboI, NdeII, Sau3AI, BstKT I
SKU SE-E187
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… ▼GATC …3'
3'… CTAG▲ …5'
Source Kurthia zopfii 9
Optimal buffer SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50100505050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by overlapping Dam methylation (G m ATC) GATC. Blocked by overlapping CG methylation C G AT m C G. Cleaved of DNA is impaired by overlapping CG methylation GAT m C G.
Reagents supplied with enzyme 10 X SE-buffer G
Notes
References
  4. S.Kh. Degtyarev, N.I. Rechkunova, A.A. Grinev, V.S. Dedkov Discovery and substrate specificity determination of restriction endonucleases Bme18I and Kzo9I. // Izvestia SB SA USSR, Biological sciences series, No.3, 25-26 (1989). (In Russian)
  5. S.Kh. Degtyarev, N.I. Rechkunova, O.A. Zhilkina, G.V. Semenchenko, M.I. Novozhilova Kurthia zopfii - a strain producer of restriction endonuclease Kzo9I // Soviet Union patent SU 1440919 (1988). (In Russian)
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