Mhl I

Enzyme nameMhl I
PrototypeSduI
Known analogsBsp1286I, SduI
SKUSE-E049
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GDGCH▼C …3'
3'… C▲HCGDG …5'
SourceMicrococcus halobius SD
Optimal bufferSE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
10257510010100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer W
NotesHigh enzyme concentration may result in star activity.
References
  1. Unpublished data Shapovalova, M.A., Abdurashitov, M.A., Dedkov, V.S., Kashirina, J.G., Popichenko, D.V., Degtyarev, S.K Unpublished observations (2000).
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