Mly113 I

Enzyme nameMly113 I
PrototypeNarI
Known analogsNarI, BbeI, Ege I, EheI, KasI, SfoI
SKUSE-E189
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GG▼CGCC …3'
3'… CCGC▲GG …5'
SourceMicrococcus lylae 113
Optimal bufferSE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
10025101050100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onT7 DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 3-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer B
NotesHigh enzyme concentration may result in star activity.
References
  1. S.V. Sosnovtsev, V.S. Dedkov, N.I. Rechkunova, Y.P. Zernov, S.Kh. Degtyarev Substrate specificity determination of restriction endonuclease Mlu113I. // Sibirian Biological Journal, No 2, 66-67 (1991). (In Russian)
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