Mox20 I

Enzyme nameMox20 I
PrototypeBalI
Known analogsBalI, MlsI, MluNI, MscI, Msp20I
SKUSE-E301
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… TGG▼CCA …3'
3'… ACC▲GGT …5'
SourceMicrobacterium oxydans
Optimal bufferSE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperatureне инактивируется
Buffer activity
BGOWYROSE
1025100752575
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lanbda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA (dcm-)
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 20-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. More complete
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityCleaved of DNA is impaired by overlapping Dcm methylation(C m CWGG) TGG CCA GG.
Reagents supplied with enzyme10 X SE-buffer O
References
// Код для некролога