MroN I

Enzyme nameMroN I
Prototype(NaeI)
Known analogsNgoMIV, NaeI, PdiI
SKUSE-E087
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… G▼CCGGC …3'
3'… CGGCC▲G …5'
SourceMicrococcus roseus N0
Optimal bufferSE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
10050100105
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onAdenovirus-2 DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme more than 90% of the Ad-2 DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer B
References
  1. Unpublished data Dedkov, V.S., Belichenko, O.A., Prikhod'ko, E.A., Abdurashitov, M.A., Degtyarev, S.K Unpublished observations (1994).
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