Nru I

Enzyme nameNru I
PrototypeNruI
Known analogsBsp68I
SKUSE-E099
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… TCG▼CGA …3'
3'… AGC▲GCT …5'
SourceNocardia rubra
Optimal bufferSE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
0107510010100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA (dam-)
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and 90% of them can be recut. In the presence of 10% PEG
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityBlocked by overlapping Dam methylation (G m ATC) TCGC GA TC.
Reagents supplied with enzyme10 X SE-buffer W
References
  2. Unpublished data Comb, D.G., Schildkraut, I., Greenough, L Unpublished observations.
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