Pce I

Enzyme name Pce I
Prototype StuI
Known analogs AatI, Eco147I, SseBI, StuI
SKU SE-E105
Turbo version Available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… AGG▼CCT …3'
3'… TCC▲GGA …5'
Source Planococcus citreus
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 50 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75755025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity Not blocked by overlapping Dcm-methylation (CmCWGG) AGG CCT GG
Reagents supplied with enzyme 10 X SE-buffer Y.
Notes At 37°C activity is 50-75% from maximum
References
  1. Unpublished data Gonchar, D.A., Shinkarenko, N.M., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K Unpublished observations (2001).
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