Pce I

Enzyme namePce I
PrototypeStuI
Known analogsAatI, Eco147I, SseBI, StuI
SKUSE-E105
Turbo versionAvailable
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… AGG▼CCT …3'
3'… TCC▲GGA …5'
SourcePlanococcus citreus
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature50 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
75755025100100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 20-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivityNot blocked by overlapping Dcm-methylation (CmCWGG) AGG CCT GG
Reagents supplied with enzyme10 X SE-buffer Y.
NotesAt 37°C activity is 50-75% from maximum
References
  1. Unpublished data Gonchar, D.A., Shinkarenko, N.M., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K Unpublished observations (2001).
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