Rga I

Enzyme name Rga I
Prototype SgfI
Known analogs AsiS I, SgfI
SKU SE-E491
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GCGAT▼CGC …3'
3'… CGC▲TAGCG …5'
Source Rhizoblum galegae
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75501025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Adenovirus-2 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity Not blocked by overlapping Dam-methylation (G m ATC) GC GATC GC Blocked by CpG methylation.
Reagents supplied with enzyme 10 X SE-buffer Y
Notes High enzyme concentration may result in star activity.
References

No publications found.
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