Izoschizomers

Rsa I

Enzyme name

Rsa I

Prototype

RsaI

Known analogs

AfaI, Csp6I

SKU

SE-E113

Turbo version

Available

High-concentration version

Not available

Restriction type

Type IIPi

Recognition site

5'… GT^▼AC …3'

3'… CA_▲TG …5'

Source

Rhodopseudomonas sphaeroides

Optimal buffer

SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)

Optimal temperature

37 °C

Inactivation temperature

не инактивируется

Buffer activity

B	G	O	W	Y	ROSE
100	50	0-10	50	75	50

Unit definition

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Assayed on

Lambda DNA

Storage conditions

10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C.

Ligation

After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Nonspecific hydrolysis

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Methylation sensitivity

not tested

Reagents supplied with enzyme

10 X SE-buffer B

References

V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Ovchinnikov bulletin of biotechnology and physical and chemical biology V.3, No 4, p. 19-27.(2007) (In Russian) 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Ovchinnikov bulletin of biotechnology and physical and chemical biology , V.2, No 3, p. 39-46 (2006). (In Russian) 2006
Y.P. Zernov, M.A. Abdurashitov, S.Kh. Degtyarev The Use of Restriction Analysis of Amplified 16S rRNA Genes for the Identification of Microorganisms: A Case Study of Bacterial Producers of Thermolabile Alkaline Phosphatase // Biotechnology, No. 6. – pp. 3–11 (2005). (In Russian) 2005
L.R. Lebedev, N.M. Pustoshilova, V.E. Repin, S.Kh. Degtyarev Isolation of restriction endonuclease RsaI from Rhodopseudomonas sphaeroides and study of its properties. // Applied biochemistry and microbiology(Moscow), Vol.27, No.3, 330-337 (1991). (In Russian) 1991
G.E. Akulin, V.E. Repin, S.Kh. Degtyarev Preparation method of restriction endonuclease Rsa I from Rhodopseudomonas sphaeroides // Soviet Union patent SU1431328 (1986). (In Russian) 1986
External publication Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F.J. Bacteriol. 142: 380-383 (1980). 1980