Rsa I

Enzyme name Rsa I
Prototype RsaI
Known analogs AfaI, Csp6I
SKU SE-E113
Turbo version Available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GT▼AC …3'
3'… CA▲TG …5'
Source Rhodopseudomonas sphaeroides
Optimal buffer SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
100500507550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer B
Notes
References
  4. L.R. Lebedev, N.M. Pustoshilova, V.E. Repin, S.Kh. Degtyarev Isolation of restriction endonuclease RsaI from Rhodopseudomonas sphaeroides and study of its properties. // Applied biochemistry and microbiology(Moscow), Vol.27, No.3, 330-337 (1991). (In Russian)
  5. G.E. Akulin, V.E. Repin, S.Kh. Degtyarev Preparation method of restriction endonuclease Rsa I from Rhodopseudomonas sphaeroides // Soviet Union patent SU1431328 (1986). (In Russian)
  6. External publication Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F.J. Bacteriol. 142: 380-383 (1980).
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