Sbf I

Enzyme name Sbf I
Prototype Sse8387I
Known analogs SdaI, Sse8387I
SKU SE-E101
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CCTGCA▼GG …3'
3'… GG▲ACGTCC …5'
Source An E.coli strain that carries the cloned Sbf I gene from Streptomyces species Bf61
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75500010050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these more than 90% canrecut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y
Notes High enzyme concentration may result in star activity.
References
  1. Unpublished data Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.Kh Unpublished observations (1994).
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