Izoschizomers

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SfaN I

Available supply options

Enzyme name

SfaN I

Prototype

SfaNI

Known analogs

LweI

SKU

SE-E165

Turbo version

Available

High-concentration version

Not available

Restriction type

Type IISi

Recognition site

5'… GCATC(N)₅^▼ …3'

3'… CGTAG(N)₉_▲ …5'

Source

An E.coli strain that carries the cloned SfaN I gene from Streptococcus faecalis N

Optimal buffer

SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Optimal temperature

37 °C

Inactivation temperature

80 °C

Buffer activity

B	G	O	W	Y	ROSE
10	25	100	75	0	25

Unit definition

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Assayed on

Lambda DNA

Storage conditions

10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.

Ligation

After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

Nonspecific hydrolysis

No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Methylation sensitivity

not tested

Reagents supplied with enzyme

10 X SE-buffer O

Notes

References

V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Ovchinnikov bulletin of biotechnology and physical and chemical biology , V.2, No 3, p. 39-46 (2006). (In Russian) 2006
Y.E. Tomilova, M.A. Abdurashitov, L.N. Golikova, N.A. Netesova, S.Kh. Degtyarev Cloning of the Genes for DNA Methyltransferases of the SfaNI and Bst19I Restriction--Modification Systems and Primary Structure Analysis of Protein Products // Molecular Biology (Moscow), Vol. 38, No.6, 997-1004 (2004). (In Russian) 2004
Y.E. Tomilova, V.S. Dedkov, N.M. Shinkarenko, D.V. Popichenko, S.Kh. Degtyarev Restriction endonuclease Bst19I from strain Bacillus stearothermophilus, that recognize DNA sequence 5′-GCATC-3′. // Biotekhnologia (Moscow), No.6, 17-20 (2002). (In Russian) 2002
G.D. Serov, S.Kh. Degtyarev, L.I. Puchkova, L.L. Korsun, N.I. Rechkunova Streptococcus faecalis - a strain producer of restriction endonuclease SfaNI. // Soviet Union patent SU 1645293 (1991). (In Russian) 1991
O.A. Zhilkina, V.E. Repin, S.Kh. Degtyarev Effect of some cultivation parameters on the yield of resctriction endonuclease SfaNI. // Applied Biochemistry and Microbiology (Moscow), Vol 27, No. 4, 486-490 (1991). (In Russian) 1991
O.A. Zhilkina, V.E. Repin, S.Kh. Degtyarev Preparation method of restriction endonuclease SfaNI, that recognizes and cleaves DNA sequence 5'-GCATC(N)5^, 3'-CGTAG(N)9^. // Soviet Union patent SU 1442546 (1988). (In Russian) 1988