SspM I

Enzyme nameSspM I
PrototypeBfaI
Known analogsMaeI
SKUSE-E591
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… C▼TAG …3'
3'… GAT▲C …5'
SourceSporosarcina species M
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature55 °C
Inactivation temperatureФермент не инактивируется прогреванием при 80°С в течение 20 минут
Buffer activity
BGOWYROSE
25-5050-7525-5050-7510080
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl. The activity at 37°C is 75% of that under standard conditions.
Assayed onLambda DNA
Storage conditions20 mM Tris-HCl (pH 7.6); 300 mM NaCl; 0.1 mM EDTA; 500 μg/ml BSA; 0.01% Triton X-100; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
LigationAfter digestion with a 3-fold excess of enzyme, 5% of DNA fragments can be ligated by DNA ligase and can be recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityBlocked by overlapping CpG methylation.
Reagents supplied with enzyme10 X SE-buffer Y AleI
References

No publications found.

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