Tru9 I

Enzyme nameTru9 I
PrototypeMseI
Known analogsMseI, Tru1I
SKUSE-E199
Turbo versionAvailable
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… T▼TAA …3'
3'… AAT▲T …5'
SourceAn E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9
Optimal bufferSE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature65 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
75252510050100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol Store at -20°C.
LigationAfter 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivityBlocked by TTA m A methylation.
Reagents supplied with enzyme10 X SE-buffer W
References
  3. S.Kh. Degtyarev, E.A. Prikhodko, N.I. Rechkunova, Y.A. Gorbunov Interaction of Vsp I and Tru9 I restriction endonuclease with synthetic oligonucleotides. // Biochimica et Biophysica Acta, 1172, 89-94 (1993).
  4. G.G. Prikhodko, N.I. Rechkunova, S.Kh. Degtyarev Substrate specificity determination of restriction endonuclease Tru9I. // Sibirian Biological Journal, No 1, 57-59 (1991). (In Russian)
// Код для некролога