Zra I

Enzyme nameZra I
Prototype(AatII)
Known analogsAat II
SKUSE-E463
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GAC▼GTC …3'
3'… CTG▲CAG …5'
SourceAn E.coli strain that carries the cloned Zra I gene from Zoogloea ramigera 11
Optimal bufferSE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
10050252575100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. In the presence of 10% PEG
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer B
NotesHigh enzyme concentration may result in star activity.The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5.ZraI cleaves linear plasmid DNA at a rate 1,5-2 times higher than supercoiled plasmid DNA.
References
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