BamH I

Description

Recognition site and hydrolysis position:

Source: An E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G + BSA
Enzyme activity (%):

Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligations:
After 50-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by Dam methylation (G^mATC) GGATCCNotes: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Wilson, G.A. and Young, F.E. J. Mol. Biol. 97: 123-125 (1975).Roberts, R.J., Wilson, G.A., Young, F.E. Nature 265: 82-84 (1977)