BamH I

1,760.00 7,040.00 

  • CNY: 143.12 ¥ - 572.49 ¥

Restriction endonuclease BamH I

  • Recognition site: G↑GATCC / CCTAG↓G
  • Источник: Из штамма E.coli несущего клонированный ген BamHI из Bacillus amyloliquefaciens H
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E021 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E021S4000 uRegular20 000 u/ml1,760.00 
  • CNY: 143.12 ¥
E022L20000 uRegular20 000 u/ml7,040.00 
  • CNY: 572.49 ¥
E021XS4000 uConcentrated50 000 u/ml1,760.00 
  • CNY: 143.12 ¥
E022XL20000 uConcentrated50 000 u/ml7,040.00 
  • CNY: 572.49 ¥
E021TS4000 uTurbo20 000 u/ml1,760.00 
  • CNY: 143.12 ¥
E022TL20000 uTurbo20 000 u/ml7,040.00 
  • CNY: 572.49 ¥

Description

Recognition site and hydrolysis position:

GGATCC G↑GATCC
CCTAGG CCTAG↓G

Source: An E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G + BSA
Enzyme activity (%):

B G O W Y ROSE
25 100 75 75 25 100

Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligations:
After 50-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by Dam methylation (GmATC) GGATCCNotes: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Wilson, G.A. and Young, F.E. J. Mol. Biol. 97: 123-125 (1975).Roberts, R.J., Wilson, G.A., Young, F.E. Nature 265: 82-84 (1977)