Description
Recognition site and hydrolysis position:
| (N)7GAAGNNNNNNTAC(N)12 | ↑(N)7GAAGNNNNNNTAC(N)12↑ |
| (N)12CTTCNNNNNNATG(N)7 | ↓(N)12CTTCNNNNNNATG(N)7↓ |
Source: Bacillus sphaericus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer 2K
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 0 | 0 | 25 | 50 | 10 | 40 |
Storage conditions: 20 mM KH2PO4(pH 7.4); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 95% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
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