Bsa29 I

3,300.00 ₽ – 13,200.00 ₽

  • CNY: 268.36 ¥ - 1,073.42 ¥

Restriction endonuclease Bsa29 I

  • Recognition site: AT↑CGAT / TAGC↓TA
  • Источник: Bacillus stearothermophilus 29
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E205 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E205S1000 uRegular20 000 u/ml3,300.00 ₽
  • CNY: 268.36 ¥
E206L5000 uRegular20 000 u/ml13,200.00 ₽
  • CNY: 1,073.42 ¥
E205TS1000 uTurbo20 000 u/ml3,300.00 ₽
  • CNY: 268.36 ¥
E206TL5000 uTurbo20 000 u/ml13,200.00 ₽
  • CNY: 1,073.42 ¥

Description

Recognition site and hydrolysis position:

ATCGAT AT↑CGAT
TAGCTA TAGC↓TA

Source: Bacillus stearothermophilus 29
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dam-)
Optimal SE-buffer: SE-buffer G + BSA
Enzyme activity (%):

B G O W Y ROSE
25 100 50 50 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G, BSA (except E205T and E206T).
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dam methylation (GmATC): GATCGATC.
Blocked by CG methylation.
Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Repin, V.E., Degtyarev, S.K. Unpublished observations.

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