Recognition site and hydrolysis position:
Source: Arthrobacter species G
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):
Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: Turbo AsiG I can be used for short time (10 min) DNA digestion as well as for standard reaction. The reaction can be performed using optimal or universal (ROSE) Buffer.
Chmuzh, E.V., Mikhnenkova, N.A., Nayakshina, T.N., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations.(2005)