Bse1 I

Bse1 I

Price range: 2 100 ₽ through 8 400 ₽

Restriction endonuclease Bse1 I

  • Recognition site:
    5'… ACTGGN▼ …3'
    3'… TGAC▲CN …5'
  • Source: Bacillus stearothermophilus 1
  • Optimal buffer: SE-buffer Y
  • Optimal temperature: 65 °C
  • Inactivated during 20 minutes at: 80 °C
SKU: SE-E035 Category:

SKUРackagePackageVariantConcentrationPrice 
E035S1000 uRegular10 000-20 000 u/ml2 100 ₽
E036L5000 uRegular10 000-20 000 u/ml8 400 ₽

Description

Recognition site and hydrolysis position:

ACTGGN ACTGGN↑
TGACCN TGAC↓CN

Source: Bacillus stearothermophilus 1
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 25 10 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme >95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Repin, V.E., Degtyarev, S.Kh. Unpublished observations.

Additional Information

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