Bse1 I

1,725.00 ₽ – 6,900.00 ₽

  • CNY: 140.28 ¥ - 561.11 ¥

Restriction endonuclease Bse1 I

  • Recognition site: ACTGGN↑ / TGAC↓CN
  • Источник: Bacillus stearothermophilus 1
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 65
  • Температура инактивации, 20 мин при: 80
SKU: SE-E035 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E035S1000 uRegular10 000-20 000 u/ml1,725.00 ₽
  • CNY: 140.28 ¥
E036L5000 uRegular10 000-20 000 u/ml6,900.00 ₽
  • CNY: 561.11 ¥

Description

Recognition site and hydrolysis position:

ACTGGN ACTGGN↑
TGACCN TGAC↓CN

Source: Bacillus stearothermophilus 1
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 25 10 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme >95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Repin, V.E., Degtyarev, S.Kh. Unpublished observations.

Related products