Bse3D I

5,500.00 22,000.00 

  • CNY: 447.26 ¥ - 1,789.04 ¥

Restriction endonuclease Bse3D I

  • Recognition site: GCAATGNN↑ / CGTTAC↓NN
  • Источник: Bacillus stearothermophilus 3D
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 60
  • Температура инактивации, 20 мин при: 80
SKU: SE-E253 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E253S200 uRegular5 000 u/ml5,500.00 
  • CNY: 447.26 ¥
E254L1000 uRegular5 000 u/ml22,000.00 
  • CNY: 1,789.04 ¥

Description

Recognition site and hydrolysis position:

GCAATGNN GCAATGNN↑
CGTTACNN CGTTAC↓NN

Source: Bacillus stearothermophilus 3D
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
10 100 25 50 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Abdurashitov, M.A., Bondar, T.S., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1996).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006