BseP I

BseP I

Price range: ₽4700,00 through ₽18800,00

Restriction endonuclease BseP I

  • Recognition site:
    5'… G▼CGCGC …3'
    3'… CGCGC▲G …5'
  • Источник: Bacillus stearothermophilus P
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 50
  • Температура инактивации, 20 мин при: 65
SKU: SE-E181 Category:

SKUРackagePackageVariantConcentrationPrice 
E181S200 uRegular5 000 u/ml₽4700,00
E182L1000 uRegular5 000 u/ml₽18800,00

Description

Recognition site and hydrolysis position:

GCGCGC G↑CGCGC
CGCGCG CGCGC↓G

Source: Bacillus stearothermophilus P
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
50 100 75 50 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation.
Notes:
References:
Langdale, J.A., Myers, P.A., Roberts, R.J. Unpublished observations (1992).

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