Bsp1720 I

Bsp1720 I

Price range: 6 500 ₽ through 26 000 ₽

Restriction endonuclease Bsp1720 I

  • Recognition site:
    5'… GC▼TNAGC …3'
    3'… CGANT▲CG …5'
  • Source: Bacillus species 1720
  • Optimal buffer: SE-buffer G
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 80 °C
SKU: SE-E185 Category:

SKUРackagePackageVariantConcentrationPrice 
E185S500 uRegular10 000 u/ml6 500 ₽
E186L2500 uRegular10 000 u/ml26 000 ₽

Description

Recognition site and hydrolysis position:

GCTNAGC GC↑TNAGC
CGANTCG CGANT↓CG

Source: Bacillus species 1720
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
50 100 50 50 75 75

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.T., Tepavicharova, I.I., Mechandjiska, L.I., Builieva, E.I. Nucleic Acids Res. 19: 2504 (1991).

Additional Information

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